Screening phytochimique et activité antifongique de Aneilema umbrosum et de Erigeron floribundus sur Aspergillus fumigatus, Penicillium sp et Candida albicans de Yaoundé

AMENDA FRANTZ MARCIA (mar007cho@yahoo.fr)
Pharmacie, Université de Yaoundé
June, 2014
 

Abstract

Introduction
The spread of the HIV/AIDS pandemic and other immunodepressing diseases has led to an
outbreak of opportunistic infections caused by fungi. Nowadays, although many antifungal
medications are available, the treatment of these infections is still a source of concern in
developing countries where the majority of the population can’t afford these drugs. Hence,
they go to traditional practitioners.
The goal of our study was to evaluate the antifungal activity of Aneilema umbrosum
and Erigeron floribundus, two plants of the Commelinaceae family and Asteraceae family
respectively.
The specific objectives were:
1- To undergo phytochemical screening of différent vegetable extract.
2- To collect, isolate and identify fungi species to be used.
3- To Select strains of Aspergillus fumigatus, Penicillium sp fungus culture collection in
the medical mycology laboratory of the FMBS.
4- Détermine inhibitory rate of extract on these species.
Methodology
The plants were collected from Mfou. Some of the fungi were from the Medical
Mycology Laboratory of the FMBS and others were isolated from patients selected at the
Mvog-Beti subdivision medical center.
This biological experimental study took place from December 2013 to April 2014 at
the Organic Chemistry Laboratory of the higher teacher training college of the University of
Yaoundé I, at the Mvog-Beti subdivision medical center, and at the Medical Mycology
Laboratory of the FMBS of the University of Yaoundé I.Erigeron floribundus, Aneilema umbrosum and all fungi species were included in our
study.
Other medicinal plants and non human species were excluded from our study.
Specimens collected from patients underwent a mycological exam in order to isolate
and identify fungal species. The study concerned 9 fungi species, including 5 Candida
albicans, 2 Aspergillus fumigatus and 2 Penicillium sp.
Extraction and phytochemical screening was done at the Organic Chemistry
Laboratory of the higher teacher training college of the University of Yaoundé I. extraction
was done by maceration in 3 solvants: methanol, dichloromethane and ethanol/water.
Result
We have noted an activity of our different extracts. This activity was range at 3 levels:
moderately active, active and very active.
Conclusion
Our results show that leaves of Erigeron floribundus and stems of Aneilema
umbrosum have an antifungal activity in vitro against yeast and filamentous fungi. Taking
into account the actual economic condition and subject to results of toxicologic tests of these
chemicals, the usage of those plants can be alternative to the medical of fungi.
Our study is an opening to more research in particular to pharmacological and
toxicological studies of these chemicals. This in collaboration with pharmacist doctor,
phytotherapeutists and medical doctor for a better use of African traditional pharmacopoeia.


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