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Abstract

RÉSUMÉ
Objectif. le but du présent travail est d’illustrer l’intérêt de l’immunohistochimie (IHC) dans la recherche étiologique des cancers colorectaux (CCR) héréditaires. Patients et Méthodes. Par la technique d’IHC, nous avons analysé 34 CCR familiaux suspectés syndrome de Lynch (SL) à partir des critères cliniques directifs de Bethesda et des pedigrees en faveur d’un mode de transmission autosomique dominant. Le diagnostic du cancer a reposé sur la clinique et l’examen anatomopathologique. Les anticorps anti-MMR : MLH1, MSH2, MSH6, PMS2 et EpCam ont été utilisés pour la détection des protéines. Résultat. Au moyen de cette technique, nous avons trouvé cinq malades soit 5% des CCR porteurs d’une immunodéficience de l’un des gènes MMR impliquant MLH1 and MSH2 en faveur du SL. Conclusion. L’IHC associée aux arbres généalogiques et aux critères cliniques, est une bonne alternative au diagnostic de certitude du SL.

ABSTRACT
Objective. The aim of our work is to illustrate the value of immunohistochemistry (IHC) in the etiological investigation of hereditary colorectal cancer (CRC). Patients and methods. We used IHC methods to analyze 34 familial CRCs suspected of Lynch syndrome (LS), according to Bethesda criteria and pedigrees pointing to autosomal dominant tranmission. The diagnosis of the cancer was based on clinical signs and pathological analysis. Anti-MMR antibodies (MLH1, MSH2, MSH6, PMS2) and Epcam were used for proteins detection. Results. Using this technique, we found five patients (5% of CCRs) carrying an immunodeficiency of one of the MMR genes involving MLH and MSH2 in favour of LS. Conclusion. IHC associated with pedigrees and clinical criteria is a good alternative for definite LS diagnosis.

Article Details

How to Cite
Poaty, H., Bolenga Liboko, A., Ondima, I., Mongo, A., Aba Gandzion, C., Pecko, J., & Gassaye, D. (2018). Place de l’Immunohistochimie dans le Diagnostic des Cancers Colorectaux Héréditaires à Brazzaville. HEALTH SCIENCES AND DISEASE, 19(3). https://doi.org/10.5281/hsd.v19i3.1092

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