Article Sidebar

Download
PDF (Français)

Main Article Content

Abstract

RÉSUMÉ
Objectif. Dans la présente étude, l’huile essentielle et les extraits bruts des écorces de Greenwayodendron suaveolens (Engl. & Diels) Verdc. subsp. suaveolens ont été testés in vitro pour leur potentiel antibactérien contre les bactéries responsable de la pneumonie. Méthodologie. Le potentiel in vitro antibactérien de l’huile essentielle et des extraits bruts a été testé en utilisant les méthodes de diffusion sur disque et de microdilution en milieu liquide. Résultats. Les résultats obtenus ont montré que les extraits testés ont une activité antibactérienne contre les agents pathogènes sélectionnés avec des diamètres de zones d’inhibition variant respectivement de 7,27 mm pour Klebsiella pneumoniae à 13,38 mm pour Haemophilus influenzae. Concernant les paramètres d’inhibition, l’huile essentielle et les extraits bruts ont également montré une activité antibactérienne avec des concentrations minimales inhibitrices comprises entre 58,59 µg/mL et 1875 µg/mL ; la plus forte activité attribuée à l’huile essentielle pour l’inhibition de la croissance de Escherichia coli. Conclusion. Les résultats de la présente investigation indiquent que les extraits des écorces de Greenwayodendron suaveolens ont le potentiel d’inhiber efficacement la croissance de Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853 et Escherichia coli ATCC 35218, responsables de la pneumonie. Cependant, l’isolement des composés bioactifs est nécessaire pour les futures investigations.

ABSTRACT
Objective. In this study, the essential oil and crude extracts from the stem-barks of Greenwayodendron suaveolens (Engl. & Diels) Verdc. subsp. suaveolens was tested for their in vitro antibacterial efficacy against pneumonia bacteria. Methodology. The essential oil and crude extracts were tested for their in vitro antibacterial potential using the disc diffusion and microdilution methods. Results. Our results showed that the tested extracts had antibacterial activity against the selected pneumonia pathogens with the zones of inhibition ranging from 7.27 mm for Klebsiella pneumoniae to 13.38 mm for Haemophilus influenzae respectively. The essential oil and crude extracts also shown antibacterial activity highlighting the minimum inhibitory concentrations (MICs) ranging from 58.59 µg/mL to 1875 µg/mL with the highest activity attributed to essential oil for inhibiting the growth of Escherichia coli. Conclusion. The present findings indicated that the extracts of stem-barks of Greenwayodendron suaveolens has the potential to inhibit effectively the growing of Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 35218, responsible of pneumonia. However, the isolation of bioactive components is a toolkit for further investigations. 

Article Details

Author Biography

PH Betote Diboue, FMBS

Medical Imaging
How to Cite
Betote Diboue, P., Nnanga, N., Benga Mekoulou, F., Obono Fouda Mballa, P., Beack Bayengue, S., Tchamgoue, D., & Nyegue, M. (2021). Effets Inhibiteurs de l’Huile Essentielle et des Extraits Bruts des Écorces de Greenwayodendron Suaveolens (Engl. & Diels) Verdc. Subsp. Suaveolens contre les Bactéries Responsables de la Pneumonie. HEALTH SCIENCES AND DISEASE, 22(3). https://doi.org/10.5281/hsd.v22i3.2572
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Hippocrates (1849). The genuine works of Hippocrates, 1:324. In: Translated from the Greek with a preliminary discourse and annotations by Francis Adams. London: Sydenham Society.
  2. Laennec R.T. (1838). A treatise on the diseases of the chest and on mediate auscultation. In: Translated from the 3rd French ed. by J. Forbes, with notes of Prof. Andral from the 4th edition. New York: SS & Wm. Wood.
  3. Rokitansky C. (1852). Inflammations of the lungs (pneumoniae). In: Manual of Pathological Anatomy. London: Sydenham Society.
  4. NICE (National Institute for Health and Care Excellence) (2014). Pneumonia in adults: diagnosis and management: Clinical guideline. Published: 3 December 2014. nice.org.uk/guidance/cg191 ; visited on December 7th 2018.
  5. Nga T. (2013). Priority Medicines for Europe and the World "A Public Health Approach to Innovation". Background Paper 6.22 Pneumonia.
  6. Mackenzie1 G. (2016). The definition and classification of pneumonia. Pneumonia. 8:14; DOI 10.1186/s41479-016-0012-z.
  7. OMS (2019). Organisation mondiale de la santé. Global Pneumonia Report 2019. https://www.who.int/fr/news-room/fact-sheets/detail/pneumonia. Visitée le 11 Janvier 2020.
  8. UNICEF (United Nations International Chlidren’s Emergency Fund) (2012). Pneumonia and diarrhoea: Tackling the deadliest diseases for the world’s poorest children. New York.
  9. UN Inter-agency Group for Child Mortality Estimation (2011). Levels and Tends in Child Mortality: Report 2011.
  10. Rudan I., Theodoratou E., Zgaga L., Nair H., Chan K.Y., Tomlinson M., et al. (2012). Setting priorities for development of emerging interventions against childhood pneumonia, meningitis and influenza. J Glob Health. 2: 010304. Medline: 23198129; doi:10.7189/jogh.01.010304.
  11. Moni N.E.D.F., Assam A.J.P., Nyegue M.A., Feudjieu E.G., Penlap B.V., Etoa F–X. (2018). Anti-mycobacterial efficacy of three essentials oils from medicinal plants currently used traditionally to treat tuberculosis in Cameroon. American Journal of Essential Oils and Natural Products. 6(3): 10-18; ISSN: 2321-9114.
  12. Mahady G.B. (2005). Medicinal Plants for the Prevention and Treatment of Bacterial Infections. Current Pharmaceutical Design. 11: 2405-2427.
  13. MacDonald I., Oghale O., Nosa O.O., Osaigbovo O. (2017). Phytochemistry, Antimicrobial and Toxicological Studies of Greenwayodendron suaveolens Seed Extracts. J Basic Pharmacol Toxicol. 1(1): 8-12.
  14. Yoo H-D., Peadar A.C., Lu Z., Eliane G., Caroline T.W., Chris M.L., Matt G.G., Mark O'Neil-J., Gary R.E., Jin-Feng H. (2005). Suaveolindole, a New Mass-Limited Antibacterial Indolosesquiterpene from Greenwayodendron suaveolens Obtained via High-Throughput Natural Products Chemistry Methods. J. Nat. Prod. 68(1) : 122-124.
  15. Tereshuck M.L., Riera M.V., Castro G.R., Abdala L.R. (1997). Antimicrobial activity of flavonoid from leaves of targets minutq. Journal of Ethnopharmacology. 56(3):227-232.
  16. CLSI. (2011). Reference method for broth dilution antifungal susceptibility testing of yeasts. Clinical and Laboratory Standards Institute, Wayne : 3-4.
  17. Brooks G.F., Butel J.S., Morse S.A. Jawetz M., Adelberg’s (2004). Medical Microbiology. 23rd Ed. International Edition, Singapore, 167-168.
  18. Cos P., Vlietinck A.J., Berghe D.V., Maes L. (2006). Anti-infective potential of natural products: How to develop a stronger in vitro proof-of-concept. Journal of Ethnopharmacology. 10(6): 290-302.
  19. Moreira M.R., Ponce A.G., de Valle C.E., Roura S.I. (2005). Inhibitory parameters of essential oils to reduce a foodborne pathogen. Lebensmittel-Wissenschaft und -Technologie-LWT. 38:565–570.
  20. Collins L., Franzblau S. (1997). Microplate alamar blue assay versus BACTEC 460 system for high-through put screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrobial Agents and Chemotherapy. 41(5): 1004-1009, ISSN 0066-4804.
  21. Fauchère J.L., Avril J.L. (2002). Bactériologie générale et médicale. Edition Ellipses. Page 365.
  22. Tekwu E.M., Pieme A.C., Beng V.P. (2012). Investigations of antimicrobial activity of some Cameroonian medicinal plant extracts against bacteria and yeast with gastrointestinal relevance. Journal of Ethnopharmacology. 142: 265-273.
  23. Li H.B., Cheng K.W., Wong C.C., Fan K.W., Chen F., Tian Y. (2007). Evaluation of antioxidant capacity and total phenolic content of different fraction of selected microalgae. Food Chemistry. 102: 771-776.
  24. Schwarz S., Noble W.C. (1999). Aspect of bacterial resistance to antimicrobial used in veterinary dermatological practice. Veterinary dermatology. 3(10): 163-176
  25. Eloff J.N. (1998). A sensitive and quick microplate method to determine the minimum inhibitory concentration of plant extracts for bacteria. Planta Medica. 64: 711-713.
  26. Olufunmiso O.O., Anthony J. A. (2012). Synergistic interactions of methanolic extract of Acacia mearnsii De wild. with antibiotics against bacteria of clinical relevance. International Journal of Molecular Sciences. 13: 8915-8932.
  27. Afagnigni A.D. (2019). Etude des propriétés antidiarrhéiques de deux plantes médicinales (Dissotis multiflora (Sm) Triana et Paullinia pinnata Linn) du Cameroun. Thèse de doctorat/Ph.D. de l’Université de Yaoundé I ; p238.

Most read articles by the same author(s)