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RÉSUMÉ
Introduction. Le Virus de l’Herpès Humain-6 (HHV-6) a été retrouvé impliqué dans l’infertilité féminine. L’objectif de notre étude était d’évaluer le profil virologique du HHV-6 et les variations cytokiniques y associées chez les femmes enrôlées pour une fécondation in vitro. Méthodologie. Une étude transversale analytique a été menée au Centre Hospitalier de Recherche et d’Application en Chirurgie Endoscopique et en Reproduction Humaine du 01 Mai 2021 au 30 Avril 2022 soit 12 mois. Au total, 92 participantes ont été inclues consécutivement. Les variables ont été comparées à l’aide d’un test de Chi² ou le test T de Student grâce au logiciel SPSS version 27 avec pour seuil statistiquement significatif, p<0,05. Résultats. Dans notre étude, 63/92 (68,5%) étaient en latence, 17/92 (18,4%) en réactivation systémique et 1/92 femme (1,1%) en réactivation locale. Aucune donnée clinique n’était associée à l’infection par le HHV-6 (p≥0,05). Du jour du transfert au jour du test de grossesse, il y avait une augmentation d’IL-10 en cas de réactivation du HHV-6 (3,66 [1,27-17,41]) et une diminution en cas de non-réactivation (-0,54 [-4,45-2,66]) (p=0,010). Par contre, les variations de l’IFN-Gamma n’étaient pas associées à un profil du HHV-6. Conclusion. La réactivation du HHV-6 systémique était retrouvée dans 2/10 cas et la locale dans 1/10 cas. Elle était associée à une augmentation de l’IL-10 du jour de transfert au jour du test de grossesse. L’impact de ce virus en Assistance Médicale à la Procréation reste à démontrer.
ABSTRACT
Introduction. Human Herpes Virus-6 (HHV-6) has been found to be involved in female infertility. The objective of our study was to evaluate the virological profile of HHV-6 and the associated cytokine variations in women enrolled for in vitro fertilization. Methodology. An analytical cross-sectional study was carried out at the Hospital Center for Research and Application in Endoscopic Surgery and Human Reproduction from May 1, 2021 to April 30, 2022, i.e. 12 months. In total, 92 participants were included consecutively. The variables were compared using a Chi square test or the Student's T test using SPSS version 27 software with a statistically significant threshold of p<0.05. Results. In our study, 63/92 (68.5%) were in latency, 17/92 (18.4%) in systemic reactivation and 1/92 women (1.1%) in local reactivation. No clinical data were associated with HHV-6 infection (p≥0.05). From the day of transfer to the day of pregnancy testing, there was an increase in IL-10 in case of HHV-6 reactivation (3.66 [1.27-17.41]) and a decrease in case of no -reactivation (-0.54 [-4.45-2.66]) (p=0.010). On the other hand, variations in IFN-Gamma were not associated with an HHV-6 profile. Conclusion. Systemic HHV-6 reactivation was found in 2/10 cases and local in 1/10 cases. It was associated with an increase in IL-10 from the day of transfer to the day of the pregnancy test. The impact of this virus on Medically Assisted Reproduction remains to be demonstrated.

Keywords

HHV-6, Epidemiology, Reactivation, Cytokine variations, In vitro fertilization HHV-6, Epidémiologie, Réactivation, Variations cytokiniques, Fécondation in vitro.

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Voundi Voundi Esther, Georges, N.-T., Claude, N. N. ., Etienne, B., Vanina, N., Serge, N., Boris, K., Sharon, M., Louise, N. M. ., Simon Stevensen, S. ., & Michel, T. (2024). Human Herpes Virus-6 and Cytokine Variations in Women Enrolled for in Vitro Fertilization: Virus de l’Herpès Humain-6 et Variations Cytokiniques chez les Femmes Enrôlées pour une Fécondation in Vitro. HEALTH SCIENCES AND DISEASE, 25(3 Suppl 1). https://doi.org/10.5281/hsd.v25i3 Suppl 1.5678
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