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Abstract

Introduction: Diabetes mellitus is a chronic metabolic disorder characterized by abnormalities in carbohydrate, lipid, and lipoprotein metabolism, which not only lead to hyperglycemia but also cause many complications, such as hyperlipidemia, hyperinsulinemia, hypertension, and atherosclerosis.

Materials and Methods: The purpose of the present study was to measure the in vitro antioxidant and biological activities of hydroethanolic extracts of Uapaca paludosa. Phytochemical screening, Antioxidant capacity (Polyphenolic content, Free radical scavenging activity, Nitric oxide scavenging activity and Metal chelating activity) and biological activities (Blood sugar assessment, Glucose tolerance test, antiamylasic activity test and Low grade postprandial inflammation) have been done.

Results: Phytochemical studies of hydroethanolic extracts of U. paludosa revealed the presence of tannins, phlobatannins, glycosids, phenols, reducing sugars, flavonoids, saponins, and alkaloids. Antioxidant activity has revealed that U. paludosa was riched in polyphenols and radical inhibitory effect was best with DPPH° radical. In glucose tolerance test, hydroethanolic extracts 400mg/kg BW allowed inhibition of glycemia than 200mg/kg BW. Hypoglycemic test revealed that hydroethanolic extracts 400mg/kg BW inhibit glycemia (6.511±1.152%) than 200mg/kg BW (0.283±0.151%). The results of low grade inflammation showed that postprandial endothelium dysfunction and oxidative stress appear 2 hours after consumption of deleterious diet. But extracts has significantly inhibited endothelium dysfunction and oxidative stress (p<0.05); U. paludosa present a reduction of postprandial nitric oxide  but only hydroethanolic extract 400mg/kg BW has presented a maximum reduction of postprandial oxidative stress (postprandial hydroperoxides).

Conclusion: Hydroethanolic extracts of U. paludosa have effect on inflammation but haven’t no effect on antiamylasic activity; because of hyperosmolar diabetis after 30min, it will be not necessary to use these extracts to cure diabetis. 

Keywords

Diabetes metabolic disorder Uapaca paludosa anti oxidant activity

Article Details

How to Cite
Tabi, O., Fokunang, C., & Tsague, M. (2013). IN VITRO ANTIOXIDANT AND BIOLOGICAL ACTIVITIES OF HYDROETHANOLIC EXTRACTS OF UAPACA PALUDOSA. HEALTH SCIENCES AND DISEASE, 13(3). https://doi.org/10.5281/hsd.v13i3.105
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