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RÉSUMÉ
Introduction. Les effets bénéfiques des antioxydants sur la santé humaine ont été largement démontrés. Aussi, des tests évaluant la capacité antioxydante du sang ont été développés. Objectifs. Mesurer l’activité antioxydante du sang par piégeage du radical libre ABTS+ sur des échantillons de sang total, de globules rouges, de plasma et de sérum. Ainsi, déterminer la fraction du sang qui présente un maximum d’activité antioxydante et qui est la plus adaptée à cette méthode d’évaluation par transfert d’électron Patients et méthodes. Cinq échantillons de sang ont été prélevés sur les auteurs de la publication dont l’âge moyen était de 35 ans. L’activité antiradicalaire a été déterminée par spectrophotométrie UV : Spectrophotomètre V-200 (BOECO, Germany). La lecture de la densité optique a été faite à 734 nm, longueur d’onde d’absorption maximale du cation radicalaire ABTS+. Résultats. Les échantillons cellulaires présentaient une activité anti radicalaire (AAR %) plus grande (sang total : 94,40 ± 0,97 ; culot globulaire : 79,14 ± 3,02) que celles du plasma (76,54 ± 1,92) ou du sérum (72,10 ± 1,71). L’AAR du sang total était supérieur de 18% à celle du plasma et de 22% à celle du sérum. La dilution des échantillons cellulaires représente une situation de stress cellulaire aux fortes dilutions (p=0,008 et p=0,014) rendant erronée la mesure de la capacité antioxydante du sang total. L’AAR du plasma était 5 à 8% supérieure à celle du sérum. Enfin, l’activité antioxydante du plasma (76,25 ± 2,88) et du sérum (70,07 ± 2,02) restait inchangée même après 7 jours au réfrigérateur. Conclusion. Nous pouvons dire que le plasma et le sérum restent les composantes du sang les plus stables et les mieux adaptés pour mesurer la capacité antioxydante totale du sang par piégeage du radical libre ABTS+, avec un léger avantage pour le plasma.
ABSTRACT
Background. The beneficial effects of antioxidants on human health have been widely demonstrated. Also, tests evaluating the antioxidant capacity of the blood have been developed. Our aim was to measure the antioxidant activity of the blood by trapping the ABTS+ free radical on samples of whole blood, red blood cells, plasma and serum. Thus, determine the fraction of the blood which presents a maximum of antioxidant activity and which is the most adapted to this method of evaluation by electron transfer Methods. Five blood samples were taken from the authors of the publication whose average age was 35 years. The anti-radical activity was determined by UV spectrophotometry: Spectrophotometer V-200 (BOECO, Germany). The reading of the optical density was made at 734 nm, wavelength of maximum absorption of the radical cation ABTS+. Results. The cell samples showed a greater antiradical activity (AAR%) (whole blood: 94.40 ± 0.97; red blood cells: 79.14 ± 3.02) than those of the plasma (76.54 ± 1.92 ) or serum (72.10 ± 1.71). The AAR of whole blood was about 18% higher than that of plasma and about 22% higher than that of serum. The dilution of cell samples represents a situation of cell stress at high dilutions (p=0.008 and p=0.014) making the measurement of the antioxidant capacity of whole blood erroneous. Plasma AAR was 5-8% higher than serum AAR. Finally, the antioxidant activity of plasma (76.25 ± 2.88) and serum (70.07 ± 2.02) remained unchanged even after 7 days in the refrigerator. Conclusion. Plasma and serum remain the most stable and best suited blood components to measure the total antioxidant capacity of blood by ABTS+ free radical scavenging, with a slight advantage for plasma.

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N’Negue ép. Mezui-Mbeng , M., Lendoye, E., Makoyo, O., Bekale, S., Ella Ndong , J., Nguema Edzang, R., & Ngou-Milama , E. (2022). Activité Antioxydante du Sang Total, du Plasma, du Sérum et des Globules Rouges par Piégeage de l’Ion Radicalaire ABTS+. HEALTH SCIENCES AND DISEASE, 23(2 Suppl 1). https://doi.org/10.5281/hsd.v23i2 Suppl 1.3429
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