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Abstract
Introduction. DNA extracted from rapid diagnostic test (RDT) cassettes can be used in molecular analysis. The objective of this study is to evaluate the effect of time, storage mode, and parasite density on Plasmodium DNA extracted from RDTs. Methodology. The study was conducted in 8 villages in the Dandé health district using RDTs made from positive thick blood smears of 19 children aged 5 to 12 years, then stored at room temperature with or without silica gel. DNA was extracted one week, two weeks, one month, and two months later. The quantity and quality of the DNA were measured using a nanodrop, followed by a standard PCR. Results. We performed 121 RDTs on our 19 participants. None of the parameters studied had a significant effect on the quantity and quality of the DNA, either individually or combined (F=2, P=0.80). The highest quantities of DNA were observed after one week of storage and with high parasite densities. Conclusion. The quality and quantity of the extracted DNA appear to be related to the storage time and parasite density.
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References
- OMS (2022) Rapport paludisme 2022. Geneva https://www.who.int/publications/m/item/WHO-UCN-GMP-2022.07
- Mbono R, Eposse C, Kamo H, et al (2023) Aspects Thérapeutiques et Évolutifs du Paludisme Grave de l ’ Enfant dans Trois Hôpitaux de Référence au Cameroun Management and Outcome of Severe Childhood Malaria in Three Reference Hospitals of Cameroon. Heal Siences Dis 24:32–36. http://www.hsd-fmsb.org/index.php/hsd
- Ishengoma DS, Lwitiho S, Madebe RA, et al (2011) Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence. Malar J 10:6. https://doi.org/10.1186/1475-2875-10-6
- Natama HM, Traoré TE, Rouamba T, et al (2023) Performance of PfHRP2-RDT for malaria diagnosis during the first year of life in a high malaria transmission area in Burkina Faso. J Parasit Dis 47:280–289. https://doi.org/10.1007/s12639-023-01566-x
- Veron V, Carme B (2006) Short report: recovery and use of Plasmodium DNA from malaria rapid diagnostic tests. 74:941–943.https://doi.org/10.4269/ajtmh.2006.74.941
- Nabet C, Doumbo S, Jeddi F, et al (2016) Analyzing Deoxyribose Nucleic Acid from Malaria Rapid Diagnostic Tests to Study Plasmodium falciparum Genetic Diversity in Mali. 94:1259–1265. https://doi.org/10.4269/ajtmh.15-0832
- Cnops L, Boderie M, Gillet P, et al (2011) Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR. 1–11. https://doi.org/10.1186/1475-2875-10-67
- Robinson A, Busula AO, Muwanguzi JK, et al (2019) Molecular quantification of Plasmodium parasite density from the blood retained in used RDTs. Sci Rep 1–8. https://doi.org/http://10.1038/s41598-019-41438-0
- Ministère de la santé du Burkina Faso (2021) Annuaire statistique 2020. Ouagadougou
- Ministère de l’économie des finances et de la prostective du Burkina Faso (2022) Annuaire statistique 2021
- Morassin B, Fabre R, Berry A, Magnaval JF (2002) One year’s experience with the polymerase chain reaction as a routine method for the diagnosis of imported malaria. Am J Trop Med Hyg 66:503–508. https://doi.org/10.4269/ajtmh.2002.66.503
References
OMS (2022) Rapport paludisme 2022. Geneva https://www.who.int/publications/m/item/WHO-UCN-GMP-2022.07
Mbono R, Eposse C, Kamo H, et al (2023) Aspects Thérapeutiques et Évolutifs du Paludisme Grave de l ’ Enfant dans Trois Hôpitaux de Référence au Cameroun Management and Outcome of Severe Childhood Malaria in Three Reference Hospitals of Cameroon. Heal Siences Dis 24:32–36. http://www.hsd-fmsb.org/index.php/hsd
Ishengoma DS, Lwitiho S, Madebe RA, et al (2011) Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence. Malar J 10:6. https://doi.org/10.1186/1475-2875-10-6
Natama HM, Traoré TE, Rouamba T, et al (2023) Performance of PfHRP2-RDT for malaria diagnosis during the first year of life in a high malaria transmission area in Burkina Faso. J Parasit Dis 47:280–289. https://doi.org/10.1007/s12639-023-01566-x
Veron V, Carme B (2006) Short report: recovery and use of Plasmodium DNA from malaria rapid diagnostic tests. 74:941–943.https://doi.org/10.4269/ajtmh.2006.74.941
Nabet C, Doumbo S, Jeddi F, et al (2016) Analyzing Deoxyribose Nucleic Acid from Malaria Rapid Diagnostic Tests to Study Plasmodium falciparum Genetic Diversity in Mali. 94:1259–1265. https://doi.org/10.4269/ajtmh.15-0832
Cnops L, Boderie M, Gillet P, et al (2011) Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR. 1–11. https://doi.org/10.1186/1475-2875-10-67
Robinson A, Busula AO, Muwanguzi JK, et al (2019) Molecular quantification of Plasmodium parasite density from the blood retained in used RDTs. Sci Rep 1–8. https://doi.org/http://10.1038/s41598-019-41438-0
Ministère de la santé du Burkina Faso (2021) Annuaire statistique 2020. Ouagadougou
Ministère de l’économie des finances et de la prostective du Burkina Faso (2022) Annuaire statistique 2021
Morassin B, Fabre R, Berry A, Magnaval JF (2002) One year’s experience with the polymerase chain reaction as a routine method for the diagnosis of imported malaria. Am J Trop Med Hyg 66:503–508. https://doi.org/10.4269/ajtmh.2002.66.503