Formulation de Gélules à Base de Feuilles et de Tiges d’Artemisia Annua

Rosine D. K. Chougouo-Nkuitchou, Alain N'guessan, Cécile Nkamenjo, Ernest Djoko, Jonas Kouamouo, Pierre Tane, Armand A. Koffi, Denis Wouessidjewe

Abstract


RÉSUMÉ
Introduction. Depuis quelques années, les Combinaisons Thérapeutiques à base d’Artémisinine (CTA) sont l’objet de nombreux cas de résistances thérapeutiques. Par ailleurs, plusieurs études ont démontré l’efficacité d’Artemisia annua (Asteraceae) sur les formes résistantes de Plasmodium. Ce travail a donc pour objectif de mettre au point des gélules à base de la poudre de feuilles et de tiges d’A. annua afin d’améliorer la prise en charge du paludisme en Afrique. Méthodes. Cette étude a consisté à caractériser la poudre et les granulés d’A. annua, obtenus par granulation humide par ajout de l’empois d’amidon de maïs. Puis des gélules dosées à 125 mg d’A. annua, ont été formulées et contrôlées selon la pharmacopée européenne 9e éd. Résultats. L’analyse granulométrique de la poudre a donné un diamètre médian (d50) inferieur à 50 µm et 600 µm pour les granulés. Les gélules obtenues, ont une répartition uniforme de la masse (125,7 mg). Le temps de désagrégation des gélules est de 3,5 ± 1,4 mn. Les teneurs en flavonoïdes totaux de la poudre et des gélules sont de 8,8  ± 1,2 mg et 1,6  ± 0,1 mg équivalent de quercétine. Conclusion. Ce travail a permis de mettre au point des gélules à base de feuilles et de tiges d’A. annua conformes à la Pharmacopée européenne 9e éd.


ABSTRACT
Introduction. In recent years, Artemisinin-based Therapeutic Combinations (CTAs) was the subject of numerous cases of therapeutic resistance. In addition, several studies have demonstrated the efficacy of Artemisia annua (Asteraceae) on the resistant forms of Plasmodium. This work therefore aims to develop capsules based on the powder of leaves and stems of A. annua to improve the management of malaria in Africa. Methods. This study consisted in characterizing the powder and granules of A. annua, obtained by wet granulation by adding corn starch paste. Then capsules containing 125 mg of A. annua, have been formulated and controlled according to the European Pharmacopoeia 9th Ed. Results. The particle size analysis of the powder gave a median diameter (d50) of less than 50 µm and 600 µm for the granules. The capsules obtained have a uniform distribution of mass (125.7 mg). The disintegration time of the capsules is 3.5 ± 1.4 min. The total flavonoid contents of the powder and capsules are 8.8 ± 1.2 mg and 1.6 ± 0.1 mg equivalent of quercétine. Conclusion. This work made it possible to develop capsules based on leaves and stems of A. annua conform to the European Pharmacopoeia 9th Ed.

Keywords


Artemisia annua, Feuilles, Tiges, Paludisme, Gélules

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