Evolution of Glycaemia in Albino Wistar Male Rats Irradiated in the Presence of Aged Garlic Extract

Brice Bertrand Kouam Foubi, Dieudonné Chuisseu Djamen, Paul Dzeufiet Djomeni, Odette Samba, Pierre Ongolo Zogo, Faustin Dong à Zok, Jean Yomi, Alain George Juimo, Samuel Nko’o Amvene, Joseph Gonsu Fotsin

Abstract


Introduction. Whole body irradiation results in blood glucose disturbances from the first hours post irradiation up to several days later. Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus; the present study was aimed at evaluating the possible protective effect of AGE on evolution of Glycaemia in Albino Wistar male rat’s post-acute whole body irradiation. Materials and Methods: Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. A slight bite to the distal tip (lateral vein) of rat tail enables to get a slight bleeding which was deposited on a reactive dipstick and the blood sugar reading was done using a glucometer.
Results. Exposure of rats to gamma irradiation caused a significant elevation of blood glucose at 4:00, 24:00, a non-significant elevation at 48:00, then a non-significant increase at 72:00 and 96:00. In rats receiving AGE orally via gavage for 5 consecutive days prior to acute irradiation and one hour after irradiation on day 6 and for 7 consecutive days, the results showed an improvement in blood glucose evolution. Conclusion. AGE seems to have protective effects against radiation-induced changes in evolution of glycaemia.
RÉSUMÉ
Introduction. L’irradiation corps entier entraîne des troubles de la glycémie dès les premiers instants après irradiation. Troubles qui peuvent se poursuivre plusieurs jours après. De nombreuses études expérimentales menées sur des animaux ont révélées que les extraits âgés d’ail possèdent diverses propriétés physiologiques. Par conséquent, la présente étude vise à évaluer l’effet protecteur probable des extraits âgés d’ail sur l’évolution de la glycémie chez les rats mâles de souche Albino Wistar post irradiation corps entier aigüe.
Matériels et Méthodes. Huit groupes constitués de cinq rats mâles ont été utilisés (20 irradiés et 20 Sham irradiés), parmi lesquels certains recevaient par voie orale de l’eau distillée, d’autres des extraits âgés d’ail à différentes doses (25 mg/kg and 50 mg/kg) et le reste de la vitamine E + de l’Acide Lipoïque. La glycémie a été réalisée en ponctionnant la veine latérale de la queue des rats. Le sang obtenu a été déposé sur une bandelette réactive et la lecture effectuée en utilisant un glucomètre. Résultats. L’exposition des rats aux irradiations gamma a entrainé une élévation significative de la glycémie à 4h00 et à 24h00, une élévation non significative à 48h00, puis une augmentation non significative à 72h00 et à 96h00. Chez les rats recevant les extraits âgés d’ail par voie orale pendant 5 jours consécutifs avant l’irradiation aigüe et une heure après irradiation le jour 6 puis pendant 7 jours consécutifs après, les résultats ont montré une amélioration concernant l’évolution de la glycémie. Conclusion. Les extraits d’ail vieilli semblent avoir des effets protecteurs concernant les dommages induits par les irradiations gamma sur l’évolution de la glycémie.


Keywords


irradiation, glycaemia, AGE, rats; radioprotection

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