Main Article Content
Abstract
Introduction. Whole body irradiation results in blood glucose disturbances from the first hours post irradiation up to several days later. Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus; the present study was aimed at evaluating the possible protective effect of AGE on evolution of Glycaemia in Albino Wistar male rat’s post-acute whole body irradiation. Materials and Methods: Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. A slight bite to the distal tip (lateral vein) of rat tail enables to get a slight bleeding which was deposited on a reactive dipstick and the blood sugar reading was done using a glucometer.
Results. Exposure of rats to gamma irradiation caused a significant elevation of blood glucose at 4:00, 24:00, a non-significant elevation at 48:00, then a non-significant increase at 72:00 and 96:00. In rats receiving AGE orally via gavage for 5 consecutive days prior to acute irradiation and one hour after irradiation on day 6 and for 7 consecutive days, the results showed an improvement in blood glucose evolution. Conclusion. AGE seems to have protective effects against radiation-induced changes in evolution of glycaemia.
RÉSUMÉ
Introduction. L’irradiation corps entier entraîne des troubles de la glycémie dès les premiers instants après irradiation. Troubles qui peuvent se poursuivre plusieurs jours après. De nombreuses études expérimentales menées sur des animaux ont révélées que les extraits âgés d’ail possèdent diverses propriétés physiologiques. Par conséquent, la présente étude vise à évaluer l’effet protecteur probable des extraits âgés d’ail sur l’évolution de la glycémie chez les rats mâles de souche Albino Wistar post irradiation corps entier aigüe.
Matériels et Méthodes. Huit groupes constitués de cinq rats mâles ont été utilisés (20 irradiés et 20 Sham irradiés), parmi lesquels certains recevaient par voie orale de l’eau distillée, d’autres des extraits âgés d’ail à différentes doses (25 mg/kg and 50 mg/kg) et le reste de la vitamine E + de l’Acide Lipoïque. La glycémie a été réalisée en ponctionnant la veine latérale de la queue des rats. Le sang obtenu a été déposé sur une bandelette réactive et la lecture effectuée en utilisant un glucomètre. Résultats. L’exposition des rats aux irradiations gamma a entrainé une élévation significative de la glycémie à 4h00 et à 24h00, une élévation non significative à 48h00, puis une augmentation non significative à 72h00 et à 96h00. Chez les rats recevant les extraits âgés d’ail par voie orale pendant 5 jours consécutifs avant l’irradiation aigüe et une heure après irradiation le jour 6 puis pendant 7 jours consécutifs après, les résultats ont montré une amélioration concernant l’évolution de la glycémie. Conclusion. Les extraits d’ail vieilli semblent avoir des effets protecteurs concernant les dommages induits par les irradiations gamma sur l’évolution de la glycémie.
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References
- G. Penelle. Description et analyse de l'accident de criticité survenu au réacteur Vénus à Mol en date du 30.12.1965.
- N. Parmentier et R. Boulenger. Problèmes de dosimétrie lors de l'accident de criticité survenu au réacteur Vénus à Mol en date du 30.12.1965.
- H.P. Jammet, R. Gongora, R. LE GO. G. Marble et M. Faes. Observation clinique et traitement d'un cas d'irradiation globale accidentelle. Proc. of the first International Congress of International Radiation Protection Association. Rome 5-10 Sept. 1966.
- Lipkan N.F. Metabolism during radiation injuries. J.P.R.S. 26.500 (1964) 17-22. [5] 68
- Vlasenko S.P., Kheytets Yu.R.The effect of ionizing radiation upon oxygen consumption and certain aspect of carbohydrate metabolism. Vopr. Radiobiol. Akad. Nouk. Arm .S.S.R. Sektor Radiobiol. Sb. (I960) 1_ 191-196.
- Graevskaya B.M.The role of hormones of the medulla of the suprarenal gland in disorders of carbohydrates metabolism due to influence of ionizing irradiation.Moscow Publish house of the Acad. of Sc. Deystvie Ionizimyushchikh Iziuchemina Organizm (1962) 157-161.
- Bresciani F., Blasif., Puca G.A. Stimulation of glycogenolysis by x-irradiation in rat liver slices in vitro : an ion-mediated effect. Radiation research (1965) 26 451-464.
- Ingram M.. Mason W.B., Hoytg.. Whipple J., Howland T.W. Biological effect of ionizing radiation. University of Rochester Report (1952) UR 196.
- Lourau-Pitres - Incorporation du 14C-Glucose dans le glycogène du foie après irradiation. Symposium de Radiobiologie de Liège 1954. Butte rworth, London (1955).
- Ganesh C. Jagetia. Radioprotective potential of plants and Herbs against the effects of Ionizing Radiation. J. Clin. Biochem. Nutr., 40, 74-81, March 2007.
- T. Tanaka, Cancer chemoprevention by natural products. Oncol. Rep.1, 1139–1155 (1994).
- T. Kong Ah-Ng, R. Yu, C. Chen, S. Mandlekar and T. Primiano, Signal transduction events elicited by natural products: Role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 23, 1–16 (2000).
- Venkatachalan S, Chattopadhyay S. Natural radioprotective agents: An overview. Curr Org Chem 2005; 9: p.389-04.
- Richard Passwater. Lipoic Acid: The Metabolic Antioxidant. McGraw-Hill; 1998.
- H. Amagase, B. L. Petesch, H. Matsuura, S. Kasuga, and Y. Itakura, “Intake of garlic and its bioactive components,” Journal of Nutrition, vol. 131, supplements 3, pp. 955S–962S, 2001.
- Balamash K, Albar O, Wang Q and Ahmed N: Effect of Kyolic® aged garlic extract on glycaemia, lipidaemia and oxidative stress in patients with type 2 diabetes mellitus. Journal of Diabetes Research and Clinical Metabolism 2012, 1:p.18.
- EEC: Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities1986; L358:1–29.
- Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen receptor. Ann Clin Biochem. 1969; 6: 24-27.
- Polikarpova L.N..Shulyatikova A. Ya. - Some changes in carbohydrate metabolism of monkeys with acute radiation sickness. A.E.C. Tr. 5430 81-88 Trad de Radiobiologya (1962) 2 390-394.
- Marble G., Frossard H., Breuil L., Engler R. - Évolution de la glycémie chez le rat après irradiation gamma. Rapport C.E.A.R. R. 2922 (1965).
- Anderson D .R..Hernandez-Diaz A., Quinn F.A. - Post-irradiation hyperglycemy in the primate.School of Aerospace Medicine, Brooks AFB (Tex) (1963).Conf 217-1 S.A. M. T.D.R. 63-40.
- Berstein E. K., Staerner, Brues - Liver function in the chick following x-irradiation.Amer. J. Physiol. (1956) 186_ 543. [24] KAY R E et ENTENMAN. Hyperglycemia and increased liver glycogen in rats after X-irradiation. Proc. Soc. Exptl. Biol. Med. 9I)143-6 1956.
- Kohn R..Ledford P., Robertson W.J. Swingley N. - Changes in plasma of the rat during fasting and influence of genetics factors upon sugar and cholesterol levels. Amer. J . Physiol. (1950) 163 410-417.
- Kohn H.. Swingley M.» Robertson W.J. - Changes in blood plasma of guinea pig during acute radiation syndrome. Amer. J. Physiol. (1951) 162 703-8.
- Data adapted by Radiation Emergency Medical management (REMM) from NATO Handbook on the Medical Aspects of NBC Defensive Operations AMedP-6(B), Chapter 6, General Medical Effects of Nuclear Weapons: Diagnosis, Treatment, and Prognosis, 1 February, 1996 .
- Raisonnier A. Évolution de la glycémie au cours du syndrome d’irradiation. Rapport français Commissariat à l’Énergie Atomique (CEA)-R3396, 1968
- Harper, H.; Radwell, V. and Mayes, P. (1977): In: Review of Physiological Chmistry. 18th ed. Lang. Medical Publication, Marzen Company Limited, Los Altos California, 328.
- Neufel.D E.F..Ginsburg V. Carbonhydrate metabolism. Ann. Rev. Biocherr.. (1965)34 297-312.
- Bresciani F., Blasif., Puca G.A. Stimulation of glycogenolysis by x-irradiation in rat liver slices in vitro : an ion-mediated effect. Radiation research (1965) 26 451-464.
- Ingram M.. Mason W.B., Hoytg.. Whipple J., Howland T.W. Biological effect of ionizing radiation. University of Rochester Report (1952) UR 196.
- Sutherland E.W.The effect of the hyperglycémie factor and epinephrine on liver and muscle phosphorylase. In: Phosphorus Metabolism (W.D. Me Elroy and B. Glass eds.) Vol.1 pp. 53-66 Johns Hopkins Press, Baltimore (1951).
- Sutherland EW, Rall TW. The relation of adenosine-3′, 5′-phosphate and phosphorylase to the actions of catecholamines and other hormones. Pharmacol. Rev. 1960;12:265.
- Ahlersova, E.; Ahlers, I. and Molecanova, A. (1988): Serum glucose and liver glycogen in gamma irradiated rats. Rad. Radiother.,29(4): 156.
- Jain R, Vyas C. Garlic in alloxan-induced diabetic rabbits.Am J Clin Nutr 1975; 28: p.684-85.
- Bordia, A. and H.C. Bansal, 1973.Essential oil of garlic in prevention of atherosclerosis. Lancet, 2: 1491-1492.
- Bordia, A.K., S.K. Sodhya, A.S. Rathore and N. Bhu, 1975.Essential oil of garlic on blood lipids and fibrinolyitc activity in patients of coronary artey disease. J. Asso. Physicians Ind., 26: 327-331.
- Jain, R.C., 1977. Effect of garlic on serum lipids, coagulability and fibrinolytic activity of blood. Am. J. Clin. Nutr., 30: 13801381.
- Sheela C, Augusti K. Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic Allium sativum Linn. Indian J Exp Biol 1992; 30:p.523-6.
- Thomson, M. and M. Ali, 2003. Garlic (Allium sativum): A review of its potential use as an anti- cancer agent. Current Cancer Drug Targets, 3: 67-81.
References
G. Penelle. Description et analyse de l'accident de criticité survenu au réacteur Vénus à Mol en date du 30.12.1965.
N. Parmentier et R. Boulenger. Problèmes de dosimétrie lors de l'accident de criticité survenu au réacteur Vénus à Mol en date du 30.12.1965.
H.P. Jammet, R. Gongora, R. LE GO. G. Marble et M. Faes. Observation clinique et traitement d'un cas d'irradiation globale accidentelle. Proc. of the first International Congress of International Radiation Protection Association. Rome 5-10 Sept. 1966.
Lipkan N.F. Metabolism during radiation injuries. J.P.R.S. 26.500 (1964) 17-22. [5] 68
Vlasenko S.P., Kheytets Yu.R.The effect of ionizing radiation upon oxygen consumption and certain aspect of carbohydrate metabolism. Vopr. Radiobiol. Akad. Nouk. Arm .S.S.R. Sektor Radiobiol. Sb. (I960) 1_ 191-196.
Graevskaya B.M.The role of hormones of the medulla of the suprarenal gland in disorders of carbohydrates metabolism due to influence of ionizing irradiation.Moscow Publish house of the Acad. of Sc. Deystvie Ionizimyushchikh Iziuchemina Organizm (1962) 157-161.
Bresciani F., Blasif., Puca G.A. Stimulation of glycogenolysis by x-irradiation in rat liver slices in vitro : an ion-mediated effect. Radiation research (1965) 26 451-464.
Ingram M.. Mason W.B., Hoytg.. Whipple J., Howland T.W. Biological effect of ionizing radiation. University of Rochester Report (1952) UR 196.
Lourau-Pitres - Incorporation du 14C-Glucose dans le glycogène du foie après irradiation. Symposium de Radiobiologie de Liège 1954. Butte rworth, London (1955).
Ganesh C. Jagetia. Radioprotective potential of plants and Herbs against the effects of Ionizing Radiation. J. Clin. Biochem. Nutr., 40, 74-81, March 2007.
T. Tanaka, Cancer chemoprevention by natural products. Oncol. Rep.1, 1139–1155 (1994).
T. Kong Ah-Ng, R. Yu, C. Chen, S. Mandlekar and T. Primiano, Signal transduction events elicited by natural products: Role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 23, 1–16 (2000).
Venkatachalan S, Chattopadhyay S. Natural radioprotective agents: An overview. Curr Org Chem 2005; 9: p.389-04.
Richard Passwater. Lipoic Acid: The Metabolic Antioxidant. McGraw-Hill; 1998.
H. Amagase, B. L. Petesch, H. Matsuura, S. Kasuga, and Y. Itakura, “Intake of garlic and its bioactive components,” Journal of Nutrition, vol. 131, supplements 3, pp. 955S–962S, 2001.
Balamash K, Albar O, Wang Q and Ahmed N: Effect of Kyolic® aged garlic extract on glycaemia, lipidaemia and oxidative stress in patients with type 2 diabetes mellitus. Journal of Diabetes Research and Clinical Metabolism 2012, 1:p.18.
EEC: Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities1986; L358:1–29.
Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen receptor. Ann Clin Biochem. 1969; 6: 24-27.
Polikarpova L.N..Shulyatikova A. Ya. - Some changes in carbohydrate metabolism of monkeys with acute radiation sickness. A.E.C. Tr. 5430 81-88 Trad de Radiobiologya (1962) 2 390-394.
Marble G., Frossard H., Breuil L., Engler R. - Évolution de la glycémie chez le rat après irradiation gamma. Rapport C.E.A.R. R. 2922 (1965).
Anderson D .R..Hernandez-Diaz A., Quinn F.A. - Post-irradiation hyperglycemy in the primate.School of Aerospace Medicine, Brooks AFB (Tex) (1963).Conf 217-1 S.A. M. T.D.R. 63-40.
Berstein E. K., Staerner, Brues - Liver function in the chick following x-irradiation.Amer. J. Physiol. (1956) 186_ 543. [24] KAY R E et ENTENMAN. Hyperglycemia and increased liver glycogen in rats after X-irradiation. Proc. Soc. Exptl. Biol. Med. 9I)143-6 1956.
Kohn R..Ledford P., Robertson W.J. Swingley N. - Changes in plasma of the rat during fasting and influence of genetics factors upon sugar and cholesterol levels. Amer. J . Physiol. (1950) 163 410-417.
Kohn H.. Swingley M.» Robertson W.J. - Changes in blood plasma of guinea pig during acute radiation syndrome. Amer. J. Physiol. (1951) 162 703-8.
Data adapted by Radiation Emergency Medical management (REMM) from NATO Handbook on the Medical Aspects of NBC Defensive Operations AMedP-6(B), Chapter 6, General Medical Effects of Nuclear Weapons: Diagnosis, Treatment, and Prognosis, 1 February, 1996 .
Raisonnier A. Évolution de la glycémie au cours du syndrome d’irradiation. Rapport français Commissariat à l’Énergie Atomique (CEA)-R3396, 1968
Harper, H.; Radwell, V. and Mayes, P. (1977): In: Review of Physiological Chmistry. 18th ed. Lang. Medical Publication, Marzen Company Limited, Los Altos California, 328.
Neufel.D E.F..Ginsburg V. Carbonhydrate metabolism. Ann. Rev. Biocherr.. (1965)34 297-312.
Bresciani F., Blasif., Puca G.A. Stimulation of glycogenolysis by x-irradiation in rat liver slices in vitro : an ion-mediated effect. Radiation research (1965) 26 451-464.
Ingram M.. Mason W.B., Hoytg.. Whipple J., Howland T.W. Biological effect of ionizing radiation. University of Rochester Report (1952) UR 196.
Sutherland E.W.The effect of the hyperglycémie factor and epinephrine on liver and muscle phosphorylase. In: Phosphorus Metabolism (W.D. Me Elroy and B. Glass eds.) Vol.1 pp. 53-66 Johns Hopkins Press, Baltimore (1951).
Sutherland EW, Rall TW. The relation of adenosine-3′, 5′-phosphate and phosphorylase to the actions of catecholamines and other hormones. Pharmacol. Rev. 1960;12:265.
Ahlersova, E.; Ahlers, I. and Molecanova, A. (1988): Serum glucose and liver glycogen in gamma irradiated rats. Rad. Radiother.,29(4): 156.
Jain R, Vyas C. Garlic in alloxan-induced diabetic rabbits.Am J Clin Nutr 1975; 28: p.684-85.
Bordia, A. and H.C. Bansal, 1973.Essential oil of garlic in prevention of atherosclerosis. Lancet, 2: 1491-1492.
Bordia, A.K., S.K. Sodhya, A.S. Rathore and N. Bhu, 1975.Essential oil of garlic on blood lipids and fibrinolyitc activity in patients of coronary artey disease. J. Asso. Physicians Ind., 26: 327-331.
Jain, R.C., 1977. Effect of garlic on serum lipids, coagulability and fibrinolytic activity of blood. Am. J. Clin. Nutr., 30: 13801381.
Sheela C, Augusti K. Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic Allium sativum Linn. Indian J Exp Biol 1992; 30:p.523-6.
Thomson, M. and M. Ali, 2003. Garlic (Allium sativum): A review of its potential use as an anti- cancer agent. Current Cancer Drug Targets, 3: 67-81.